Core Facility Core Facility for Mass Spectrometry & Proteomics (CFMP)

Services

Regular Services:

• Molecular weight determination of intact proteins 
• Protein identification of gel separated proteins 
• Identification of interaction partners from Co-IP experiments 
• Analysis of proximity labeling (BioID) experiments 
• Proteome analysis 

Additional services (detailed discussion before is a prerequisite):

• Analysis of post- translational modifications (e.g. phosphorylation, succinylation)
• Identification of cross- linked peptides of purified proteins and protein complexes
• Cell- wide or organelle- wide interactome analysis (co- fractionation MS)
• Please, contact us for additional application that may fit your needs.

Equipment

Mass spectrometers:

• Q-Exactive HF MS (Thermo) coupled with Dionex Ultimate 3000 RSLCnano (Thermo)
• Qtrap 5500 MS (ABSciex) coupled with Dionex Ultimate 3000 RSLCnano (Thermo)
• maXis UHR-TOF (Bruker Daltonics) coupled with Infinty LC (Agilent)

Additional equipment:

• 1D (Invitrogen)
• Pipetting robot Digest Pro (Intavis)
• Ultimate 3000 RSLC Offline LC System equiped with an automated fraction collector (Thermo)
• Capillary laser puller P-2000F (Sutter Instruments)
• Vanquish Neo (Thermo)

Additional information about our Equipment can be found here:

Equipment

A detailed price list including project examples can be found here. Please, note that the final price has to be discussed with the head of the CFMP.

Each facility user is obliged to be familiar and sign CFMP user regulations.  

The contribution of the CFMP to research projects should be properly acknowledged. This acknowledgment not only enhances the visibility of the CFMP but also supports its efforts in securing external funding and acquiring new instruments. Contributions from the CFMP may include, but are not limited to:  

• Development of new analytical methods  
• Assistance with experimental design  
• Data generation and/or evaluation  
• Support in manuscript preparation, including figure creation, preparation of supplementary data, and submission of raw data. 

Samples are processed on a first come first serve basis. Sample processing includes protein digestion into peptides, sample clean-up, peptide separation and quantification using LC-MS and an initial database search. Regular services are usually processed usually within 2-4 weeks. Additional services usually take up to 8 weeks.