Ti-Crest spinning disk microscope

Spinning disk system with 7 laser lines that allows to remove the spinning disk so that it may also be used in widefileld. In contrast to the Ti-VoX, the Ti-Crest supports all capabilities of Nikon's NIS Elements software as well as Nikon's Perfect Focus System (PFS).

Microscope

Fully motorized Nikon Ti2. Water dispenser for long term imaging with water immersion objectives available.

Software

Nikon NIS-Elements

Confocal Scanning Unit

Crest X-Light V3

Confocal light source

Lumencor Celesta Light Engine
Available excitation laser lines:

• 405 nm
• 440 nm
• 488 nm
• 514 nm
• 561 nm
• 640 nm
• 730 nm

Cameras

Andor Zyla-4.2P

• 4.2 Megapixels (2048 x 2048)
• Pixel size 6.5 x 6.5μm
• FOV 18mm
• Sensor Area 13.3mm x 13.3mm
• Quantum Efficiency up to 82%
• 53 fps (USB 3.0), 100 fps (Camera Link)

Photometrics Iris 15

• 15 Megapixels (5056 x 2960)
• Pixel size 4.25 x 4.25μm
• FOV 25mm
• Sensor Area 21.49mm x 12.61mm
• Quantum Efficiency up to 73%
• Up to 30 fps

 

Available objectives (subject to change)

• Nikon Plan Apo
  10x 0.45 λS
  DIC N1 WD 4.0
  coverslip thickness: 0.17mm


• Nikon Plan Apo VC
  20x 0.75
  DIC N2 WD 1.0
  coverslip thickness: 0.17mm


• Nikon Plan Apo 25x 1.05 SIL λS
  DIC N2 WD 0.55
  coverslip thickness correction: 0.11–0.23mm
  Silicone Oil Immersion (n=1.40)


• Nikon Plan Apo
  40x 1.25 SIL λS
  DIC N2 WD 0.3
  coverslip thickness correction: 0.13–0.21mm
  Silicone Oil Immersion (n=1.40)


• Nikon Plan Apo λ
  60x 1.40 OIL
  DIC N2 WD 0.13
  coverslip thickness: 0.17mm
  Oil Immersion (n=1.515)


• Nikon Plan Apo IR
  60x 1.25 WI
  DIC N2 WD 0.16 – 0.18
  coverslip thickness: 0.15 – 0.19mm
  Water Immersion (n=1.33)

Bearbeiter: Christian Ackermann

Latest Revision: 2022-05-07