LaVision Ultramicroscope II SPIM

Profile: Lightsheet microscope for imaging of cleared large specimens (up to 30x30x15mm) in up to three channels.

After the installation of a gas exhaust system the microscope is now available. The laser lines are 488, 561 and 640 nm.
The Ultramicroscope II is a light sheet microscope that is especially made for big specimen like mice brains. Imaging of such large structures requires optical clearing.
At the moment we have two objectives:
1. LaVision LVMI 4x NA 0.3 objective with a working distance of 6 mm. The refractive index can be adjusted in the range from 1.30 to 1.60.
2. LaVision MI Plan 12x NA 0.53 objectice with a working distance of 10.9 mm. The refractive index can be adjusted from 1.374 to 1.667.
The camera is an Andor Neo sCMOS wit 5.5 Mpixel and a pixel size of 6.5 µm. High resolution images may be acquired by stitching smaller image fields.

LaVision Ultramicroscope II

Wound healing assay Nikon A1R
Mouse embryo at stage 14.5
acquired with the Ultramicroscope II
Image: Nicolas Dross
Sample courtesy of Maximilian Schönung, AG Lipka


Bearbeiter: Christian Ackermann

Latest Revision: 2020-02-12

zum Seitenanfang/up