Barrier

The barrier areas 2-8 are exclusively for housing and breeding of transgenic mice and rats and have therefore been assigned a S1-classification.

To ensure a constantly high hygienic standard, following measures are necessary.

• Access to the barrier is restricted. Entry is only possible after a gating process.
• All material which is brought into the barrier has to be autoclaved or disinfected.
• New mouse lines (especially from other institutions) must submit to a sanitation process before they can be bred in the barrier.

Housing	Mouse	Rat
Type of facility	SPF barrier	SPF barrier
cage type	II & II L, conventional	IV, conventional
Bedding	ABEDD LT-E-001	ABEDD LT-E-001
number of cage companions	see recommendations GV-SOLAS	see recommendations GV-SOLAS
Humidity	50-60%	50-60%
Light-dark cycle	12-12	12-12
Temperature	22°C +/- 2°C	22°C +/-2°C
type of food	Altromin Rod 16 or Rod 18	Altromin Rod 16 or Rod 18
access to food	ad libitum	ad libitum
environmental enrichment	yes, Crincklets Nest-Pads	yes, polycarbonate huts