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Do you want to know more?
Responsible
Frank Zimmermann
54-5433 / 54-5705

Sascha Dlugosz
54-6883 / 54-5705

 
Here you can get our porcedure catalogue as a PDF-file.

 
Here you can find all further forms.
 

Biotechnology lab

General

You can find more info about our different services by clicking on the tab "Procedures". These services can also be ordered by external clients provided there are capacities.

Here you can find our forms.

Procedures

PN / Cytoplasm / Perivittelin microinjection

  • Mouse (positive foanders not guaranteed).
  • Injection of min 200 C57Bl/6NCRL Oocytes, other strains on demand
  • Oviducttransfer in 10 recipients
  • Rat (positive foanders not guaranteed).
  • Injection of min 200 Spraque Dawley oocytes, other strains on demand.
  • Ampulla transfer in 10 recipients

ES Injection

  • Mouse (chimeras not guaranteed).
  • Injection of min 30 C57Bl/6NCRL blastocysts per clone, other strains on demand, Uterustransfer in 3 recipients.
  • Alternativ: Injection of 60 Blastozysten per day, Uterustransfer in 6 recipients

Co-culture Chimeras

  • Co-culture of ES cells: Mouse (chimeras not guaranteed).
  • Aggregation of 60 C57Bl/6NCRL or FVB/N Morulae per clone. Other strains on demand.
  • Uterustransfer in 4-6 recipients

Cryopreservation of embryos

  • Cryopreservation of embryos in 2-cell stadium (Mouse and Rat)
  • Cryopreservation of 200 2-cells per strain/line in Minitübs of 20 2-cells aliquots.
  • Storage in two different buildings ander strictly monitored conditions in liquid nitrogen at -196°C (connected to central technical control room, on-call duty).)

Cryopreservation of sperm (Mouse)

  • Cryopreservation of sperm from 2-5 males of strain/line in 5 Minitübs per male.
  • Storage in two different buildings ander strictly monitored conditions in liquid nitrogen at -196°C (connected to central technical control room, on-call duty).)

Revitalization

Revitalization of embryos from the IBF embryobank of the BTL: Mouse and Rat
  • Thawing of 1-2 Minitübs approx. 20- 40 embryos (depending on strain the results of the embryos are at 90-98%. Generally 30-60% of transfered embryos are born. Oviducttransfer in 3-4 recipients.

Revitalization of embryos, which were not stored in the IBF embryobank: Mouse/Rat.
  • Embryos are thawed according to protocol, and transfered into recipients via embryotransfer. We can provide no guarantee, mostly due to unknown conditions of cryopreservation and storage. Very often also the case for quality and quantity.

Strain/line-sanitization (Mouse and Rat)

  • Collection of 2-cells from contaminated strain/line.
  • Oviduct (Mouse)/ampulla transfer (Rat) into SPF foster mothers (SPF Unit)
  • Mikrobiologal examination of foster mothers after weaning of pups

In Vitro fertilisation (IVF)

In-vitro-fertilisation mouse for “PROPAGATION“ of non breeding males: Mouse.
  • Collection of sperm from cauda epididymis
  • Fertilisation of oocytes from 15-20 C57BL/6NCRL females, other strains on demand.
  • Cultivation until 2-cell stadium
  • Oviducttransfer/Cryopreservation of developed 2-cells.

In-vitro-Fertilisation Mouse for the “PRESERVATION“ of several identical genotypes: Mouse.
  • Collection of sperm from cauda epididymis
  • Fertilisation of oocytes from 15-20 C57BL/6NCRL females, other strains on demand.
  • Cultivation until 2-cell stadium
  • Oviducttransfer of developed 2-cells.

If necessary, a laser-assisted IVF is going to be done.

Intracytoplasmatic sperm injection (ICSI)

ICSI: Mouse and Rat
  • Collection of sperm from cauda epididymis of living males (in case of dead males not after 12 hours post mortem)
  • Injection of spermheads into oocytes
  • Cultivation until 2-cell stadium
  • Oviducttransfer into recipients

Preimplantation diagnostics (PID)

PID: Mouse
  • Biopsy of zygotes in 4 t 8-cell stadium
  • Individual cultivation (until blastocyst) until diagnosis.
  • Uterustransfer of selected blastocysts

Lentiviral Transgenesis

LTG: Mouse/Rat
  • Injection, incubation of min 200 oocytes. Strains on demand.
  • Oviducttransfer into 10 recipients

Ovartransplantation (Mouse/Rat)

  • Collection of donor ovaries
  • Transplantation of donor ovaries in immunodeficient recipients
  • Mating until pregnancy

Computer Assisted Sperm Analysis (CASA)

  • after 14 days of sexual abstinence. Removal of both Cauda epididymis (CE), terminal or surgical
  • CE are immersed in 500µl pre-incubated M-HTF beneath paraffin oil
  • CE are opened (sperm float)
  • Computer assisted analyisi starts after 60 mins.

Bildergalerie

 

Laser-assisted ES-cell Injection


Es Laserunterstuetzte Es 1 Es Laserunterstuetzte Es 2 Es Laserunterstuetzte Es 3


 

Chimeras from laser-assisted ES-cell injection

from E14 ES-cells from R1 ES-cells
Es Chimaeren Aus Laser Es E1
 
Es Chimaeren Aus Laser Es R2

 

PN-Injection

PN-Injection Mouse PN-Injection Rat
Pn Injektion Maus
Pn Injektion Ratte

 

Lentiviral TG


Lenti Tg

In Vitro Fertilisation (IVF): Mouse

In vitro fertilisation of mouse oozyte 6-10 hourse after insemination (pronucleus state) 24 hours after insemination (2 cell stage)
Ivf Maus 1
Ivf Maus 2
Ivf Maus 3

Laserassisted intra-cytoplasmastic spermheadinjection


140211_ICSI

Ovartransplantation Mouse

Otm 1
Otm 2
Otm 3
Otm 4
Otm 5

Strain/line sanitization via embryotransfer into the SPF unit

2 cell stage from contaminated superovulated donors After collection and several washes Ovidukttransfer int pseudo-pregnant (0.5 days) foster mothers
Sanierung 1
Sanierung 2
Sanierung 3

Cryopreservation of 2-Zellern mittels "Two-Step-Methode"


Kryokonservierung

 
Latest Revision: 2017-08-02
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