Current Research

Cellular porcine model

Patch-clamp and histochemistry of porcine dorsol root ganglion cells to characterize different nociceptor classes Development of in-vitro model of nerve endings separated from the cell body and coculture with keratinocytes.

Porcine single fiber recordings

Characterization of different afferent nerve fiber classes and modulation of axonal excitability using standard teased fiber techniques in the pig saphenous nerve Effects of locally applied growth factors on excitability of nerve endings and axonal excitability.

Porcine neurogenic inflammation.

Development of experimental models of peripheral sensitization by UVB irradiation with the objective readout of axon reflex erythema measured by laser Doppler imaging. Microdialysis in the sunburn to assess levels of inflammatory mediators involved in peripheral sensitization.

Human pain models

Characterization of human pain models for peripheral (UVB irradiation) and central (electrical pain and hyperalgesia model) sensitization by pharmacological intervention Objective assessment of cutaneous unmyelinated innervation by electrically induced axon reflex erythema and electrically induced axon reflex sweating.

Chronic pain patients

Microneurographic characterization of neuropathic changes in primary afferent fibers linked to chronic pain Correlation of functional and structural changes in neuropathic pain by using skin biopsies, quantitative sensory testing, and electrically induced axon reflex measurements.

Schmelz Fig1
Fig. 1: Two-compartment in vitro model of piglet DRG neurons with somata and processes separated by a diffusion barrier. Microphotographs after 6 days in cell culture. PGP9.5 staining.

 

Schmelz Fig2
Fig. 2: Corresponding patterns of activity dependent slowing of conduction velocity (360 stimuli at 2Hz, followed by 40 stimuli at 0.25Hz) in respective C-fiber classes in humans (microneurography) and pig (teased fiber technique). cmi: mechano-insensitive nociceptors, cm: polymodal nociceptors, symp: sympathetic efferent, cold: cold nociceptors
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Latest Revision: 2012-08-01
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