Nikon/Andor Ti-TuCam microscope
Profile: Inverted widefield fluorescence microscope withe sensitive b/w-camera, an automated stage and an on-stage incubator (temperature/5% CO2) that can hold multiwell plates and smaller dishes.
Ti2-TuCam
The Nikon/Andor TuCam system is a fully automated Nikon Ti2 microscope with an Andor Neo sCMOS camera with 2560 x 2160 (5.5M) pixels, a pixel area of 6.5 x 6.5 μm, and a well count of 30,000 electrons. It can be cooled to -40°C and offers a sustained readout rate of 30 frames per second (100 fps burst rate).
Neuronal growth cone imaged in Ti-TuCam
Optics:
- Nikon Plan Fluor 10x NA 0.3 (working distance 15.2 mm), corrected for 0.17mm coverslips (= type #1.5).
- Nikon S Plan Fluor ELWD 20x NA 0.45 (working distance 8.2 - 6.9mm) adjustable for glas and plastic bottom dishes with a thickness between 0 and 2 mm).
- Nikon Plan Apo VC 20x NA 0.75 (working distance 1 mm), corrected for 0.17mm coverslips (= type #1.5).
- Nikon Apo LWD 40x NA 1.15 λS water immersion objective (working distance 0.59 - 0.61mm), adjustable for coverslips from 0.15 - 0.19mm thickness.
- Nikon Plan Apo VC 60x NA 1.4 oil immersion objective (working distance 0.13mm), corrected for 0.17mm coverslips (= type #1.5).
- Nikon Plan Fluor 100x NA 1.3 oil immersion objective (working distance 0.16mm), corrected for 0.17mm coverslips (= type #1.5).
Filters:
- ex 390/22 - bs 420 - em 460/50 ("DAPI")
- ex 438/24 - bs 458 - em 483/32 ("CFP")
- ex 472/30 - bs 495 - em 520/35 ("EGFP")
- ex 500/24 - bs 520 - em 542/27 ("YFP")
- ex 562/40 - bs 593 - em 624/40 ("TxRed")
- ex 632/22 - bs 660 - em 692/40 ("Cy5")
Fluorescence light sources:
For normal widefield fluorescence:Lumencor Sola SE 5-LCR-SB
For DMD:
Lumencor Spectra
Environmental control:
Onstage incubator from TokaiHit. Suitable for multiwell plates of 127.8 mm × 85.5 mm size or smaller dishes.
