Nikon Ti-HCS microscope

Profile: High content screening system for multiwell plates in phase contrast and/or fluorescence.

Here we have a fully automated, inverted Nikon Ti2 microscope made for high content screening tasks. The microscope is encased by a environmental box from Oko Lab for temperature-, CO2- and humidity control. By a software module names "JOBS" in the Nikon NIS-Elements acquisition software it is possible to screen in x/y, z and t everything from a LabTek dish to a multiwell plate. Images can be taken in brightfield and epifluorescence.


U2OS cells expressting TagRFP-Actin imaged in the Nikon Ti-HCS system

Objectives

  • Nikon Plan Fluor 4x NA 0.13 (working distance 16.5mm)
  • Nikon Plan Fluor 10x NA 0.3 phase contrast (working distance 16mm; corrected for 0.17mm coverslips (= type #1.5))
  • Nikon Plan Fluor 20x NA 0.75 Multi Immersion (working distance 0.35mm; water, glycerol. oil; adjustable for glas bottom dishes of type #1 - 2)
  • Nikon S Plan Fluor ELWD 20x NA 0.45 (working distance 8.2 - 6.9 mm; adjustable for glas and plastic bottom dishes with a thickness between 0 and 2mm)
  • Nikon S Plan Fluor ELWD 40x NA 0.60 (working distance 3.6 - 2.8mm; adjustable for glas and plastic bottom dishes with a thickness between 0 and 2mm)

Camera

Nikon DS-Qi2 high-sensitive CMOS monochrome camera with 2404 x 2404 pixel resolution (in total 4908×3264, the image is cropped to fit the FOV of the microscope) and a quantum efficiency of 80% at 500nm.
With the DS-Qi2 camera at 2404 x 2404 pixel resolution, the following pixel sizes result: 4x 1.83µm; 10x 0.73µm; 20x 0.37µm; 40x 0.18µm.

Fluorescent light source

Lumencor Sola SE II

 

Bearbeiter: Christian Ackermann

Latest Revision: 2018-05-08

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