Nikon Ti-HCS microscope
The Ti-HCS is a fully automated, inverted Nikon Ti2 microscope made for high content screening tasks. The microscope is encased by a environmental box from OkoLab for temperature-, CO2- and humidity control. By a software module names "JOBS" in the Nikon NIS-Elements acquisition software it is possible to screen in x/y, z and t everything from a LabTek dish to a multiwell plate. Images can be taken in brightfield and epifluorescence.
U2OS cells expressting TagRFP-Actin imaged in the Nikon Ti-HCS system
Objectives
- Nikon Plan Fluor 4x NA 0.13 (working distance 16.5mm)
- Nikon Plan Fluor 10x NA 0.3 phase contrast (working distance 16mm; corrected for 0.17mm coverslips (= type #1.5))
- Nikon Plan Apo 20x NA 0.75 (working distance 1.0mm)
- Nikon S Plan Fluor ELWD 20x NA 0.45 (working distance 8.2 - 6.9 mm; adjustable for glas and plastic bottom dishes with a thickness between 0 and 2mm)
- Nikon Plan Apo 40x NA 0.95 (working distance 0.25 - 0.17mm)
- Nikon S Plan Fluor ELWD 40x NA 0.60 (working distance 3.6 - 2.8mm; adjustable for glas and plastic bottom dishes with a thickness between 0 and 2mm)
Camera
Nikon DS-Qi2 high-sensitive CMOS monochrome camera with 2404 x 2404 pixel resolution (in total 4908×3264, the image is cropped to fit the FOV of the microscope) and a quantum efficiency of 80% at 500nm.
With the DS-Qi2 camera at 2404 x 2404 pixel resolution, the following pixel sizes result: 4x 1.83µm; 10x 0.73µm; 20x 0.37µm; 40x 0.18µm.
Fluorescent light source
Lumencor Sola SE II
